| 说明： |
Dissolve ingredients (21.09g/L) and sparge medium with 100% N 2 gas for 30–45min to make it anoxic. Add cysteine and adjust pH to 7.0, then dispense medium under 100% N 2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Adjust pH of complete medium to 6.8-7.0, if necessary. |
