Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Phoenix is a second-generation retrovirus producer cell line for the generation of helper-free ecotropic and amphotropic retroviruses.
The unique feature of this cell line is that it is highly transfectable with either calcium phosphate mediated transfection or lipid-based transfection protocols-- up to 50% or higher of cells can be transiently transfected.
This cell line is capable of carrying episomes for long-term stable production of retrovirus (LZRS).
Phoenix Ecotropic (ECO) cell line expresses ecotropic envelope protein.
CD8 expression is a direct reflection of intracellular gag-pol and the stability of the producer cell population's ability to produce gag-pol can be readily monitored by flow cytometry.
Phoenix-ECO have been extensively tested for helper virus production and established as being helper-virus free.
Note: Culture to ≤80% confluence to prevent the cells from sloughing
Swift S et al. Rapid Production of Retroviruses for Efficient Gene Delivery to Mammalian Cells Using 293T Cell-Based Systems. Current Protocols in Immunology,Unit 10.17C, 2001
Pear WS, et al. Production of High-Titer Helper-Free Retroviruses by Transient Transfection. Proc. Natl. Acad. Sci. USA 90: 8392-8396, 1993. PubMed: 7690960
Nolan Laboratory Retroviral Systems. Phoenix helper-free retrovirus producer lines, https://web.stanford.edu/group/nolan/_OldWebsite/retroviral_systems/retsys.html
