293T B161444_ATCC代理_宁波明舟生物科技有限公司，细胞，菌株，ATCC，保藏中心

293T

规格:

价格:

编号:B161444

品牌:Mingzhoubio

标准菌株

定量菌液

DNA

RNA

规格:

冻干粉

斜面

甘油

平板

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产品名称

293T

商品货号

B161444

Organism

Homo sapiens, human

Tissue

embryonic kidney

培养基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

2 [Cells contain Adenovirus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age

fetus

Storage Conditions

liquid nitrogen vapor phase

Images

Derivation

The 293T cell line, originally referred as 293tsA1609neo, is a highly transfectable derivative of human embryonic kidney 293 cells, and contains the SV40 T-antigen.

Comments

This cell line is competent to replicate vectors carrying the SV40 region of replication. It gives high titers when used to produce retroviruses. It has been widely used for retroviral production, gene expression and protein production.

Complete Growth Medium

The base medium for this cell line is Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC 30-2002). To make the complete growth medium, add the following components to the base medium: 10% Fetal Bovine Serum (heat inactivated) (ATCC 30-2020), 2mM L-glutamine (ATCC 30-2214)

Subculturing

Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.05% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.

Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended.
Medium renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C
Atmosphere:air, 95%; carbon dioxide (CO₂)

STR Profile

CSF1PO: 11,12
D13S317: 12,14
D16S539: 9,13
D5S818: 8,9
D7S820: 11
TH01: 7, 9.3
TPOX: 11
vWA: 16,19
Amelogenin: X

Name of Depositor

Stanford Univ.

References

DuBridge RB, et al. Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system. Mol. Cell Biol. 7: 379-387, 1987. PubMed: 3031469

Pear WS, et al. Production of high-titer helper-free retroviruses by transient transfection. Proc. Natl. Acad. Sci. USA. 90: 8392-8396, 1993. PubMed: 7690960

B191181：293T/17 SF [HEK 293T/17 SF]

B161450：293T/17 [HEK 293T/17]

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