| 产品名称 | NCI-H1568 [H1568] |
|---|---|
| 商品货号 | B161439 |
| Organism | Homo sapiens, human |
| Tissue | lung; derived from metastatic site: lymph node |
| Product Format | frozen |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | adenocarcinoma; non-small cell lung cancer |
| Age | 48 years |
| Gender | female |
| Ethnicity | Caucasian |
| Images |  |
| Derivation | The line was established in December 1986 from lymph node metastasis of lung. |
| Clinical Data | 48 years
Caucasian female The patient was a smoker. 60 pack years. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Culture medium, 95%; DMSO, 5% |
| Culture Conditions | Temperature: 37°C |
| STR Profile | Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 10,12 D5S818: 9 D7S820: 8,9 THO1: 9 TPOX: 8,11 vWA: 16,17 |
| Name of Depositor | AF Gazdar, JD Minna |
| Deposited As | Homo sapiens |
| Year of Origin | 1986 |
| References | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. |
| 梅经理 | 17280875617 | 1438578920 |
| 胡经理 | 13345964880 | 2438244627 |
| 周经理 | 17757487661 | 1296385441 |
| 于经理 | 18067160830 | 2088210172 |
| 沈经理 | 19548299266 | 2662369050 |
| 李经理 | 13626845108 | 972239479 |
