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HCM-BROD-0106-C71
HCM-BROD-0106-C71
规格:
货期:
编号:B161434
品牌:Mingzhoubio

标准菌株
定量菌液
DNA
RNA

规格:
冻干粉
斜面
甘油
平板


产品名称 HCM-BROD-0106-C71
商品货号 B161434
Organism Homo sapiens, human
Tissue brain
Product Format frozen 1.0 mL
Morphology neurosphere
Culture Properties aggregate/suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Recurrent glioblastoma
Age See associated clinical data for patient profile information, if available.
https://portal.gdc.cancer.gov/  
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71
Gender See associated clinical data for patient profile information, if available.
https://portal.gdc.cancer.gov/  
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71
Ethnicity See associated clinical data for patient profile information, if available.
https://portal.gdc.cancer.gov/  
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71
Applications Basic research, compound screening.
Storage Conditions liquid nitrogen vapor phase
Images
Clinical Data

ICD-10-CM code: C71, malignant neoplasm of cerebrum

See associated clinical data for patient profile information, if available.
https://portal.gdc.cancer.gov/  
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71
Comments Next-generation cancer model from the Human Cancer Models Initiate (HCMI). Refer to the following websites for additional information on this model including protocols, clinical information, and bioinformatics data.

https://ocg.cancer.gov/programs/hcmi/resources
https://portal.gdc.cancer.gov/  
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71

Complete Growth Medium

NeuroCult NS-A Basal Medium (StemCell Technologies #05750) with NS-A Proliferation Supplement (StemCell Technologies #05754) + 20 ng/mL EGF (StemCell Technologies #78003.1) + 20 ng/mL bFGF (Peprotech #AF-100-15) + 2 µg/mL Heparin (StemCell Technologies #07980).

Prepare media according to the manufacturer’s instructions: Stem Cell Technologies Catalog #5751

Subculturing Important: use Ultra Low Attachment (ULA) flasks/plates when culturing this model such as Corning #3814.
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2.5-5 x 105 cells/mL and maintain between below 1 x 106 cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). Large neurospheres should be mechanically dissociated to small fragments by repeated pipetting. Do not dissociate to single cells.
Cryopreservation Complete growth media containing 10% DMSO (ATCC 4-X)
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Cells per Vial ≥ 1 x 106 cells
Volume 1.0 mL
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 9
D16S539: 9,13
D5S818: 12,13
D7S820: 9,11
THO1: 7,9.3
TPOX: 8,11
vWA: 15,16
Sterility Tests Bacteria, yeast and fungi: No growth
Mycoplasma: No growth
Viral Testing Hepatitis B: None detected
Cytomegalovirus: None detected
Human immunodeficiency virus: None detected
Epstein-Barr virus: None detected
Human papillomavirus: None detected
Name of Depositor Broad Institute
Year of Origin 2018
梅经理 17280875617 1438578920
胡经理 13345964880 2438244627
周经理 17757487661 1296385441
于经理 18067160830 2088210172
沈经理 19548299266 2662369050
李经理 13626845108 972239479