产品名称 |
HCM-BROD-0106-C71 |
商品货号 |
B161434 |
Organism |
Homo sapiens, human |
Tissue |
brain |
Product Format |
frozen 1.0 mL |
Morphology |
neurosphere |
Culture Properties |
aggregate/suspension |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease |
Recurrent glioblastoma |
Age |
See associated clinical data for patient profile information, if available. https://portal.gdc.cancer.gov/ https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71
|
Gender |
See associated clinical data for patient profile information, if available. https://portal.gdc.cancer.gov/ https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71
|
Ethnicity |
See associated clinical data for patient profile information, if available. https://portal.gdc.cancer.gov/ https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71
|
Applications |
Basic research, compound screening. |
Storage Conditions |
liquid nitrogen vapor phase |
Images |
|
Clinical Data |
ICD-10-CM code: C71, malignant neoplasm of cerebrum
See associated clinical data for patient profile information, if available. https://portal.gdc.cancer.gov/ https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71
|
Comments |
Next-generation cancer model from the Human Cancer Models Initiate (HCMI). Refer to the following websites for additional information on this model including protocols, clinical information, and bioinformatics data.
https://ocg.cancer.gov/programs/hcmi/resources
https://portal.gdc.cancer.gov/
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0106-C71
|
Complete Growth Medium |
NeuroCult NS-A Basal Medium (StemCell Technologies #05750) with NS-A Proliferation Supplement (StemCell Technologies #05754) + 20 ng/mL EGF (StemCell Technologies #78003.1) + 20 ng/mL bFGF (Peprotech #AF-100-15) + 2 µg/mL Heparin (StemCell Technologies #07980).
Prepare media according to the manufacturer’s instructions: Stem Cell Technologies Catalog #5751 |
Subculturing |
Important: use Ultra Low Attachment (ULA) flasks/plates when culturing this model such as Corning #3814.
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2.5-5 x 105 cells/mL and maintain between below 1 x 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Large neurospheres should be mechanically dissociated to small fragments by repeated pipetting. Do not dissociate to single cells. |
Cryopreservation |
Complete growth media containing 10% DMSO (ATCC 4-X) |
Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
Cells per Vial |
≥ 1 x 106 cells |
Volume |
1.0 mL |
STR Profile |
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 9
D16S539: 9,13
D5S818: 12,13
D7S820: 9,11
THO1: 7,9.3
TPOX: 8,11
vWA: 15,16 |
Sterility Tests |
Bacteria, yeast and fungi: No growth
Mycoplasma: No growth |
Viral Testing |
Hepatitis B: None detected
Cytomegalovirus: None detected
Human immunodeficiency virus: None detected
Epstein-Barr virus: None detected
Human papillomavirus: None detected
|
Name of Depositor |
Broad Institute |
Year of Origin |
2018 |